Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes.

Synthesis of DNA complementary to 14S calf lens crystallin messenger RNA by reverse transcriptase.

Lens differentiation. Crystallin synthesis in isolated epithelia from calf lenses

In the calf eye lens, four morphologically distinct cell types can be detected: three in the epithelial monolayer and one in the cortical part. During differentiation, there is a quantitative change in the synthesis of crystallin subunits. A marked increase in alphaA-chains and several beta-crystallin polypeptides accompanies the transition from epithelial to fiberlike lens cells while synthesi...

Isolation and in vitro translation of delta-crystallin mRNA from embryonic chick lens fibers.

Of the protein synthesized and accumulated during differentiation of embryonic chick lens fibers, 70-80% is the tissue specific protein delta-crystallin. We have isolated and partially characterized the total cytoplasmic mRNA from purified lens fibers of 15-day-old embryos as an initial step toward understanding the regulated expression of delta-crystallin during development. Each lens fiber ma...

Thermal Stress Induced Aggregation of Aquaporin 0 (AQP0) and Protection by α-Crystallin via Its Chaperone Function

Aquaporin 0 (AQP0) formerly known as membrane intrinsic protein (MIP), is expressed exclusively in the lens during terminal differentiation of fiber cells. AQP0 plays an important role not only in the regulation of water content but also in cell-to-cell adhesion of the lens fiber cells. We have investigated the thermal stress-induced structural alterations of detergent (octyl glucoside)-solubil...

Quantitation of delta-crystallin messenger RNA during lens induction in chick embryos.

Embryonic chick cells of the presumptive lens ectoderm are induced to differentiate into a lens by the optic vesicle, an outgrowth from the developing brain. This lens induction involves formation of the lens placode by cell elongation between about 44 and 50 hr of development, followed by invagination of the placode between about 50 and 55 hr of development. The amount of delta-crystallin mess...